{"id":2483,"date":"2024-12-11T11:53:38","date_gmt":"2024-12-11T14:53:38","guid":{"rendered":"https:\/\/posgraduacao.icc.fiocruz.br\/?page_id=2483"},"modified":"2024-12-11T11:53:39","modified_gmt":"2024-12-11T14:53:39","slug":"diferenciais","status":"publish","type":"page","link":"https:\/\/posgraduacao.icc.fiocruz.br\/?page_id=2483","title":{"rendered":"Diferenciais"},"content":{"rendered":"<div class=\"wpb-content-wrapper\"><p>[vc_row][vc_column width=&#8221;1\/2&#8243;][vc_custom_heading text=&#8221;Cursos Internacionais PPGBB&#8221; font_container=&#8221;tag:h2|font_size:50|text_align:left|line_height:1&#8243; google_fonts=&#8221;font_family:Lato%3A100%2C100italic%2C300%2C300italic%2Cregular%2Citalic%2C700%2C700italic%2C900%2C900italic|font_style:400%20regular%3A400%3Anormal&#8221;][vc_empty_space][vc_column_text]<span style=\"font-size: 22px;\">Os Cursos Internacionais PPGBB t\u00eam como objetivo intensificar o desenvolvimento da internacionaliza\u00e7\u00e3o e a excel\u00eancia da educa\u00e7\u00e3o no Programa de<br \/>\nP\u00f3s-gradua\u00e7\u00e3o em Bioci\u00eancias e Biotecnologia, e contribuir para o processo de internacionaliza\u00e7\u00e3o da p\u00f3s-gradua\u00e7\u00e3o stricto sensu e da ci\u00eancia, tecnologia e inova\u00e7\u00e3o.<\/span>[\/vc_column_text][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_single_image image=&#8221;1507&#8243; img_size=&#8221;full&#8221;][vc_empty_space][\/vc_column][\/vc_row][vc_row type=&#8221;vc_default&#8221; full_width=&#8221;stretch_row&#8221; css=&#8221;.vc_custom_1664375203256{background: rgba(30,115,190,0.28) url(https:\/\/posgraduacao.icc.fiocruz.br\/wp-content\/uploads\/2022\/09\/barra-contato-1.png?id=1513) !important;*background-color: rgb(30,115,190) !important;}&#8221;][vc_column width=&#8221;1\/2&#8243;][\/vc_column][vc_column width=&#8221;1\/2&#8243;][vc_custom_heading text=&#8221;Entre em contato conosco&#8221; font_container=&#8221;tag:h2|font_size:50|text_align:left|color:%23ffffff|line_height:1&#8243; google_fonts=&#8221;font_family:Lato%3A100%2C100italic%2C300%2C300italic%2Cregular%2Citalic%2C700%2C700italic%2C900%2C900italic|font_style:400%20regular%3A400%3Anormal&#8221;][vc_empty_space][vc_btn title=&#8221;ensino.icc@fiocruz.br&#8221; style=&#8221;flat&#8221; shape=&#8221;square&#8221; size=&#8221;lg&#8221; i_icon_fontawesome=&#8221;fa fa-envelope&#8221; add_icon=&#8221;true&#8221;][vc_btn title=&#8221;+55 41.3316-3233&#8243; style=&#8221;flat&#8221; shape=&#8221;square&#8221; size=&#8221;lg&#8221; i_icon_fontawesome=&#8221;fa fa-phone-square&#8221; add_icon=&#8221;true&#8221;][vc_empty_space height=&#8221;40px&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_custom_heading text=&#8221;Cursos anteriores&#8221; font_container=&#8221;tag:h2|font_size:50|text_align:left|line_height:1&#8243; google_fonts=&#8221;font_family:Lato%3A100%2C100italic%2C300%2C300italic%2Cregular%2Citalic%2C700%2C700italic%2C900%2C900italic|font_style:400%20regular%3A400%3Anormal&#8221;][\/vc_column][\/vc_row][vc_row][vc_column][vc_tta_accordion color=&#8221;peacoc&#8221; active_section=&#8221;0&#8243;][vc_tta_section title=&#8221;International Course on Bioinformatics Analysis of Human Whole Exome Sequencing Data&#8221; tab_id=&#8221;1692197658678-d816b99e-d91a&#8221;][vc_column_text]<strong>Coordinator<\/strong>: Fabio Passetti<\/p>\n<p><strong>Collaborating Professors<\/strong>: Dr. Luc\u00eda Spangenberg and Dr. Hugo Naya from the Institut Pasteur de Montevideo<\/p>\n<p><strong>Course Load<\/strong>: 24 hours<\/p>\n<p><strong>Period<\/strong>: November 22 to 24, 2022<\/p>\n<p><strong>Schedule<\/strong>: Tuesdays and Fridays, from 1:00 PM to 3:00 PM<\/p>\n<p><strong>Syllabus<\/strong>:<\/p>\n<ul>\n<li><strong>Day 1<\/strong>: Introduction to Next-Generation Sequencing (NGS); mapping and quality analysis using Linux and R; and mapping with calibration of Whole Exome Sequencing (WES) data.<\/li>\n<li><strong>Day 2<\/strong>: Identification of genetic polymorphisms; types of genetic variants and quality analysis; and practical session with GATK software for identifying genetic polymorphisms in WES data.<\/li>\n<li><strong>Day 3<\/strong>: Epidemiology applied to genetics; population genomics; and exercises with case discussions.<\/li>\n<li>Em Combining induced pluropotent &#8230;<\/li>\n<\/ul>\n<p>&nbsp;[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Combining induced pluripotent stem cells and structural approaches to study neurodegenerative diseases&#8221; tab_id=&#8221;1692197658679-eacfbe84-a86b&#8221;][vc_column_text]<strong>Coordinator<\/strong>: Tatiana de Arruda Campos Brasil de Souza<\/p>\n<p><strong>Collaborating Professor<\/strong>: Dr. Helen Cristina Miranda (Case Western University)<\/p>\n<p><strong>Course Load<\/strong>: 30 hours<\/p>\n<p><strong>Period<\/strong>: October 24 \u2013 28, 2022<\/p>\n<p><strong>Schedule<\/strong>: 9:00 AM &#8211; 5:00 PM<\/p>\n<p><strong>Syllabus<\/strong>:<\/p>\n<p><strong>Module I<\/strong><br \/>\nAvailable from October 17, 2022: Theoretical and practical classes on the use of visualization and manipulation programs for three-dimensional structures such as PyMOL, Chimera, etc. Analysis of protein-protein interactions. Online program for three-dimensional structure modeling.<\/p>\n<p><strong>Module II<\/strong><br \/>\nOctober 24 \u2013 Theoretical classes on basic concepts of stem cells, isolation, culture, characterization, and differentiation of adult and pluripotent stem cells.<\/p>\n<p>October 26, 2022 \u2013 How to model neurological diseases using 2D and 3D human stem cell cultures.[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Genomic Biomarkers and their Interpretation in Hematologic Malignancies&#8221; tab_id=&#8221;1696941563350-57f4f7b3-fe3a&#8221;][vc_column_text]<\/p>\n<div>\n<p><strong>Invited professors<\/strong><\/p>\n<\/div>\n<div>\n<ul>\n<li><span class=\"x_ContentPasted0 x_ContentPasted1\">Adam C. Smith \u2013 University of Toronto <\/span><\/li>\n<li>Jos\u00e9-Mario Capo-Chichi &#8211; University of Toronto<\/li>\n<li>Hubert Tsui \u2013 University of Toronto<\/li>\n<\/ul>\n<\/div>\n<p>[\/vc_column_text][vc_column_text]<\/p>\n<div>\n<p><strong>Invited professors<\/strong><\/p>\n<\/div>\n<div>\n<ul>\n<li><span class=\"x_ContentPasted0 x_ContentPasted1\">Adam C. Smith \u2013 University of Toronto <\/span><\/li>\n<li>Jos\u00e9-Mario Capo-Chichi &#8211; University of Toronto<\/li>\n<li>Hubert Tsui \u2013 University of Toronto<\/li>\n<\/ul>\n<\/div>\n<p>[\/vc_column_text][vc_column_text]<\/p>\n<div class=\"x_elementToProof x_ContentPasted1\"><strong>Provide students with an overview of the classification of hematological malignancies and the techniques capable of detecting the genetic alterations associated with these diseases.<\/strong><\/div>\n<div class=\"x_elementToProof x_ContentPasted1\"><\/div>\n<ul>\n<li class=\"x_elementToProof x_ContentPasted1\"><span class=\"x_ContentPasted2\">Understand the updated classification of hematologic malignancies launched in 2022 by the World Health Organization and the International Consensus Classification.<\/span><\/li>\n<li class=\"x_elementToProof x_ContentPasted1\"><span class=\"x_ContentPasted4\">&nbsp;Discuss the differences and problems related to the diagnosis of these diseases.<\/span><\/li>\n<\/ul>\n<div class=\"x_elementToProof x_ContentPasted1\">\n<ul data-editing-info=\"{&quot;orderedStyleType&quot;:1,&quot;unorderedStyleType&quot;:2}\">\n<li><span class=\"x_ContentPasted4 x_ContentPasted5\">Review the use of cytogenetics and cytogenomic techniques to detect structural variants. Understand and apply the International Standard for Cytogenetic Nomenclature (ISCN) in practical cases and scenarios. Review cytogenetic techniques related to diagnosis.<\/span><\/li>\n<\/ul>\n<\/div>\n<div class=\"x_elementToProof x_ContentPasted1\">\n<ul data-editing-info=\"{&quot;orderedStyleType&quot;:1,&quot;unorderedStyleType&quot;:2}\">\n<li><span class=\"x_ContentPasted4 x_ContentPasted6\">Review the techniques used in Molecular Genetics laboratories.<\/span><\/li>\n<li><span class=\"x_ContentPasted4 x_ContentPasted6 x_ContentPasted7\">Compare the advantages and disadvantages of various techniques.<br aria-hidden=\"true\"><\/span><\/li>\n<\/ul>\n<\/div>\n<div class=\"x_elementToProof\">\n<ul data-editing-info=\"{&quot;orderedStyleType&quot;:1,&quot;unorderedStyleType&quot;:2}\">\n<li><span class=\"x_ContentPasted8\">Use the&nbsp;<span class=\"x_ContentPasted9\">acquired<\/span>&nbsp;knowledge in specific objectives 1-3 for application in practical cases and scenarios.<br aria-hidden=\"true\"><\/span><\/li>\n<li class=\"x_ContentPasted10\">Discussion of testing approaches in &#8220;resource-rich&#8221; and &#8220;resource-poor&#8221; environments. Investigate new applications for genetic testing in Onco-Hematology, including proposing hereditary leukemias and other special topics.<\/li>\n<\/ul>\n<\/div>\n<p>[\/vc_column_text][vc_column_text]<strong>Part 1<\/strong><\/p>\n<ul>\n<li class=\"x_ContentPasted11\">\n<div class=\"x_ContentPasted12\">Basic concepts of Biology of Cancer and Hematological Neoplasms: 8 hours<\/div>\n<\/li>\n<li class=\"x_ContentPasted11\">\n<div class=\"x_ContentPasted12\">Genomic Basis of Hematological Neoplasms: 4 hours<\/div>\n<\/li>\n<li class=\"x_ContentPasted11\">\n<div class=\"x_ContentPasted12\">WHO and ICC classification of Hematological Neoplasms: 4 hours<\/div>\n<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_column_text]<strong>Part 2<\/strong><\/p>\n<ul>\n<li>Cytogenetic and Molecular Methods Molecular Nomenclature (HGNC): 2 hours<\/li>\n<li>\n<div class=\"x_ContentPasted16\">Cytogenetic Nomenclature (ISCN) : HGNC and ISCN Nomenclature Workshop : 4 hours<\/div>\n<\/li>\n<\/ul>\n<p>[\/vc_column_text][vc_column_text]<strong>Part 3<\/strong><\/p>\n<ul>\n<li class=\"x_ContentPasted16 x_ContentPasted17 x_ContentPasted19\">New Generation of Biomarker Testing and characterization of Acquired Hematologic Neoplasms Using NGS: 4 hours<\/li>\n<li class=\"x_ContentPasted16 x_ContentPasted17 x_ContentPasted19 x_ContentPasted20\">Optical genome mapping (OGM) applied to Hematological Neoplasms: 4 hours<\/li>\n<li class=\"x_ContentPasted16 x_ContentPasted17 x_ContentPasted21\">Workshop \u2013 Case examples with report using mock NGS and GMO results: 4 hours<\/li>\n<li class=\"x_ContentPasted16 x_ContentPasted17 x_ContentPasted22\">Additional Topics of Interest: What is CHIP and CCUS (Clonal Hematopoesis of Interdeterminant Potential and Clonal Cytopenia of Undetermined Significance): 2 hours<\/li>\n<li class=\"x_ContentPasted16 x_ContentPasted17 x_ContentPasted22\">Hereditary Predisposition to Hematologic Neoplasms:&nbsp; Case Discussion: 4 hours<\/li>\n<\/ul>\n<p>[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;Integration of Structural Biology Approaches for Studying Macromolecules&#8221; tab_id=&#8221;1708370236084-1bbf074e-91ca&#8221;][vc_column_text]<span style=\"color: #000000;\">The aim of this workshop is to give some tips and tools to researchers interested in studying the structure-function relationships in macromolecules and macromolecular complexes. To get a deep understanding of a biological process, an integration between biophysics, biochemistry, bioinformatics and structural biology is important. It is also important to stress the fact that one cannot be expert in each and every field, but each expertise is important to build the puzzle. Nevertheless, having an idea of which are the possibilities, and the possible pitfalls of several techniques will be fundamental to choose the right collaboration and to better follow their approaches and results. Therefore, I shall present some of the techniques with specific examples, also in relationships with the possibilities offered by Synchrotrons.<\/span><\/p>\n<p>&nbsp;<\/p>\n<h4 class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" data-placeholder=\"Tradu\u00e7\u00e3o\" data-ved=\"2ahUKEwif8fO74_qBAxWHppUCHdbaCygQ3ewLegQICBAQ\"><span style=\"color: #000000;\"><strong><span class=\"Y2IQFc\" lang=\"en\">Responsible teacher<br \/>\n<\/span><\/strong><\/span><\/h4>\n<p><span style=\"color: #000000;\">Adriana Erica Miele &#8211; <span class=\"Y2IQFc\" lang=\"en\">teacher <\/span><span class=\"Y2IQFc\" lang=\"en\">of Biochemistry and Biophysics at the University Claude Bernard Lyon 1. Lyon, <\/span><\/span><\/p>\n<p><span style=\"color: #000000;\">Researchgate: https:\/\/www.researchgate.net\/profile\/Adriana-Miele<\/span><\/p>\n<h4 id=\"tw-target-text\" class=\"tw-data-text tw-text-large tw-ta\" dir=\"ltr\" data-placeholder=\"Tradu\u00e7\u00e3o\" data-ved=\"2ahUKEwif8fO74_qBAxWHppUCHdbaCygQ3ewLegQICBAQ\"><strong><span class=\"Y2IQFc\" lang=\"en\">&nbsp;<\/span><\/strong><\/h4>\n<ul>\n<li><span style=\"color: #000000;\"><strong>The theoretical classes will be offered in a hybrid format only for participants outside of Curitiba.<\/strong><\/span><\/li>\n<li><span style=\"color: #000000;\"><strong>The practical class will be limited to 18 participants. Those interested in the practical class should indicate their intention by sending a text justifying their interest.<\/strong><\/span><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><span style=\"color: #000000;\">Registration for the practical class &#8211; <a style=\"color: #000000;\" href=\"https:\/\/forms.gle\/ogxMegXnao4eC4cYA\" target=\"_new\" rel=\"noopener noreferrer\">https:\/\/forms.gle\/ogxMegXnao4eC4cYA<\/a><\/span><\/p>\n<h3><span style=\"color: #000000;\">Monday October 30th 2023<\/span><\/h3>\n<p><strong><span style=\"color: #000000;\"><em>9:00-10:30 \u2013 Integrative structural biology<\/em><\/span><\/strong><\/p>\n<p><span style=\"color: #000000;\">An introduction on the different methods that can be used to gain structural information on macromolecules, with emphasis on Microscopy (super resolution, atomic force and electron microscopies), NMR,&nbsp; crystallography and small angle X-ray scattering (SAXS)<\/span><\/p>\n<p><strong><span style=\"color: #000000;\"><em>11:00-12:30 \u2013 cryoElectron Microscopy (cryoEM)<\/em><\/span><\/strong><\/p>\n<p><span style=\"color: #000000;\">A quick tour on the theory and practice of EM, including sample preparation and a few examples of applications.<\/span><\/p>\n<p>&nbsp;<\/p>\n<h3><span style=\"color: #000000;\">Break<\/span><\/h3>\n<p><strong><span style=\"color: #000000;\"><em>14:00-15:30 \u2013 Tutorial on SAXS<\/em><\/span><\/strong><\/p>\n<p><span style=\"color: #000000;\">EMBL-Hamburg offers the package ATSAS for data analysis online as well as for download. The tutorial will show how to analyse protein SAXS data and which information can be obtained.<\/span><\/p>\n<h3><\/h3>\n<p>&nbsp;<\/p>\n<h3><span style=\"color: #000000;\">Tuesday October 31st 2023<\/span><\/h3>\n<p><strong><span style=\"color: #000000;\"><em>9:00-10:30 \u2013 How to read a structure paper: a hitchhiker guide for non structuralists<\/em><\/span><\/strong><\/p>\n<p><span style=\"color: #000000;\">Seeing is believing, but which are the possible pitfalls to avoid if you are not a specialists? A guided tour on a few examples.<\/span><\/p>\n<p><strong><span style=\"color: #000000;\"><em>11:00-12:00 \u2013 When structure meets function (or not)<\/em><\/span><\/strong><\/p>\n<p><span style=\"color: #000000;\"><em>Schistosoma mansoni<\/em> is a complex multicellular parasite and an interesting biological puzzle, needing a multi-technical approach to be tackled.<\/span>[\/vc_column_text][\/vc_tta_section][vc_tta_section title=&#8221;In vitro and in vivo systems to study the immune response to pathogens and vaccines: Hepatitis B virus and Bacillus Calmette-Gu\u00e9rin models&#8221; tab_id=&#8221;1733922856479-7bf02322-f750&#8243;][vc_column_text]<span style=\"color: #000000;\">The analysis of the immunological response to different pathogens represents an important tool for understanding the pathogenesis of infections, as well as for the development of vaccines and therapies.<\/span><\/p>\n<p><span style=\"color: #000000;\">One of the fundamental aspects of the immune response is the recognition of foreign invaders and the development of an immune response. At the cellular level, immune cells can sense pathogen associated molecular patterns (PAMPs) and start an immune response at the cellular level. Specifically for viral pathogens, cells sense the pathogens using different receptors and initiate the modulation of intrinsic cells factors and pathways (like type I IFN) which present antiviral activity. Understanding the role of these pathways may lead to the development of antiviral strategies, either specific or broadly to multiple viruses.<\/span><\/p>\n<p><span style=\"color: #000000;\">Additionally, from a systemic point of view one important characteristic of the immune response is the ability of immune cells to migrate to the site of infection, or even to circulate through different immune organs (lymph nodes, spleen and thymus). This property of immune cells is responsible for capturing non-self antigens in the periphery, initiating specific immune responses in immune tissues. Therefore, analyzing how this cell migration occurs <em>in vivo<\/em> is relevant to understand the dynamics of the immune response to antigens. Furthermore, this knowledge can be useful for the development of more efficient vaccines, or for the treatment of immunological disorders associated with cell migration to inflamed tissues.<\/span><\/p>\n<p><span style=\"color: #000000;\">For some pathogens these studies are quite limited, due to the handling biosafety level, or the difficulty of cultivating those microorganisms <em>in vitro<\/em> and\/or <em>in vivo<\/em>. The hepatitis B virus (HBV) represents a classic example of a pathogen in which the <em>in vitro<\/em> and <em>in vivo<\/em> cultivation of the agent represents one of the main challenges. Although there is a safe and efficient vaccine for preventing acute and chronic infection with the virus, curing chronically infected patients still represents a key challenge. <em>In vitro<\/em> and <em>in vivo<\/em> culture systems for this pathogen, although useful, still have major limitations. Additionally, studies involving the infection\/transfection of susceptible cells (HepG2 or Huh7.5 expressing the sodium taurocholate co-transporting peptide, NTCP receptor) have brought some advances, but there are still important limitations. Therefore, the course will focus on <em>in vitro<\/em> cultivation of HBV on HepG2-NTCP cells and the recently described HBV pregenomic RNA (pgRNA) system to study HBV biology and the antiviral immune response. Also, the Bacillus Calmette-Gu\u00e9rin (BCG), the vaccine against tuberculosis, will be used to study cell migration using protocols that allow cell tracking and enable the understanding of the migration dynamics of different cells <em>in vivo<\/em>.<\/span><\/p>\n<p><span style=\"color: #000000;\">Finally, students will have the opportunity to learn about new tools and protocols for studying the immune response against HBV and BCG, which can be adapted to other pathogens of interest.<\/span><\/p>\n<h3><\/h3>\n<h3>Program<\/h3>\n<p><strong>Day 1 (10\/28\/2024)<\/strong><\/p>\n<p><strong>9:00-10:30h &#8211; Theoretical class I \u2013 General aspects of HBV biology <\/strong><\/p>\n<p>Lefteris Michailidis, PhD \u2013 Emory University, Atlanta, USA.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>11:00-12:00h \u2013 Protocol Discussion I \u2013 Virus stock preparation, infection of HepG2-NTCP cells with HBV, and a cell culture system supporting the complete life cycle of HBV <\/strong><\/p>\n<p>Lefetris Michailidis, PhD \u2013 Emory University, Atlanta, USA<\/p>\n<p>&nbsp;<\/p>\n<p><strong>13:30-18:00h \u2013 Practical class I \u2013 Stock preparation, infection of HepG2-NTCP cells with HBV <\/strong><\/p>\n<p>Lefetris Michailidis, PhD \u2013 Emory University, Atlanta, USA<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Day 2 (10\/29\/2024)<\/strong><\/p>\n<p><strong>9:00-10:30h &#8211; Theoretical class II \u2013 Primary human hepatocytes as a model to study HBV biology and innate immune responses<\/strong><\/p>\n<p>Lefteris Michailidis, PhD \u2013 Emory University, Atlanta, USA<\/p>\n<p>&nbsp;<\/p>\n<p><strong>11:00-12:00h \u2013 Protocol Discussion II \u2013 Targeted arrayed overexpression and CRISPR KO screens for virus-host interaction studies in the context of HBV infection<\/strong><\/p>\n<p>Lefteris Michailidis, PhD \u2013 Emory University, Atlanta, USA<\/p>\n<p>&nbsp;<\/p>\n<p><strong>13:30-18:00h \u2013 Practical class II \u2013 Targeted arrayed overexpression and CRISPR KO screens for virus-host interaction studies in the context of HBV infection<\/strong> Lefteris Michailidis, PhD \u2013 Emory University, Atlanta, USA<\/p>\n<p><strong>&nbsp;<\/strong><strong>&nbsp;<\/strong><\/p>\n<p><strong>Day 3 (10\/30\/2024)<\/strong><\/p>\n<p><strong>9:00-10:30h &#8211; Theoretical class III \u2013 An RNA-based system to study HBV replication and evaluate antivirals<\/strong><\/p>\n<p>Yingpu Yiu, PhD \u2013 Rockefeller University, New York, USA.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>11:00-12:00h \u2013 Protocol Discussion III \u2013 Transfection of susceptible cells with pgRNA or HBV DNA plasmids<\/strong><\/p>\n<p>Yingpu Yiu, PhD \u2013 Rockefeller University, New York, USA.<\/p>\n<p><strong>&nbsp;<\/strong><\/p>\n<p><strong>13:30-18:00h \u2013 Practical class III \u2013 Transfection of susceptible cells with pgRNA or HBV DNA plasmids<\/strong><\/p>\n<p>Yingpu Yiu, PhD \u2013 Rockefeller University, New York, USA.<\/p>\n<p><strong>&nbsp;<\/strong><\/p>\n<p><strong>Day 4 (10\/31\/2024)<\/strong><\/p>\n<p><strong>9:00-10:30h &#8211; Theoretical class IV \u2013 HBV readouts for studying HBV biology, virus-host interactions and antivirals<\/strong><\/p>\n<p>Yingpu Yiu, PhD \u2013 Rockefeller University, New York, USA.<\/p>\n<p><strong>&nbsp;<\/strong><\/p>\n<p><strong>11:00-12:00h \u2013 Protocol Discussion IV \u2013 HBV quantification on the supernatant of cell cultures by Real Time PCR and analysis of the expression of HBV S antigen by Flow Cytometry<\/strong><\/p>\n<p>Yingpu Yiu, PhD \u2013 Rockefeller University, New York, USA.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>13:30-18:00h \u2013 Practical class IV \u2013 HBV quantification on the supernatant of cell cultures by Real Time PCR and analysis of the expression of HBV S antigen by Flow Cytometry<\/strong><\/p>\n<p>Yingpu Yiu, PhD \u2013 Rockefeller University, New York, USA.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Day 5 (11\/01\/2024)<\/strong><\/p>\n<p><strong>9:00-10:30h &#8211; Theoretical class V \u2013 Deep mutation scanning of hepatitis B virus reveal a mechanism for cis-preferential reverse transcription (virtual class)<\/strong><\/p>\n<p>William Schneider, PhD \u2013 Rockefeller University, New York, USA.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>11:00-12:30h \u2013 General discussion \u2013 How to choose the right laboratory for a Postdoc?<\/strong><\/p>\n<p>Yingpu Yiu, PhD \u2013 Rockefeller University, New York, USA.<\/p>\n<p>Lefteris Michailidis, PhD \u2013 Emory University, Atlanta, USA (virtual)<\/p>\n<p>William Schneider, PhD \u2013 Rockefeller University, New York, USA (virtual)<\/p>\n<p><strong>&nbsp;<\/strong><\/p>\n<p><strong>14:30-16:30h \u2013 Theoretical class VI \u2013 Discussion on the experimental results and protocols<\/strong><\/p>\n<p>Yingpu Yiu, PhD \u2013 Rockefeller University, New York, USA.<\/p>\n<p>Lefteris Michailidis, PhD \u2013 Emory University, Atlanta, USA (virtual)<\/p>\n<p>&nbsp;<\/p>\n<p><strong>16:30h \u2013 18:00h \u2013 Happy hour<\/strong><\/p>\n<p>&nbsp;<\/p>\n<p><strong>Suggested reading for the first week of the course:<\/strong><\/p>\n<p><strong>&nbsp;<\/strong><\/p>\n<p>Tsukuda S, Watashi K. Hepatitis b virus biology and life cycle. Antiviral Res 2020, 182:104925. doi: 10.1016\/j.antiviral.2020.104925<\/p>\n<p>&nbsp;<\/p>\n<p>Seeger C, Mason WS. Molecular biology of hepatitis B virus infection. Virology 2015, 479-480:673:86. doi: 10.1016\/j.virol.2015.02.031<\/p>\n<p>&nbsp;<\/p>\n<p>Michailidis E, et al. A robust cell culture system supporting the complete life cycle of hepatits B virus. Scientific Report 2017, 7(1):16616<strong>. <\/strong>doi: 10.1038\/s41598-017-16882-5<\/p>\n<p>&nbsp;<\/p>\n<p>Michailidis E, et al. Expansion, in vivo-ex vivo cycling, and genetic manipulation of primary human hepatocytes. Proc Natl Acad Sci USA 2020, 117(3):1678-1688. doi: 10.1073\/pnas.1919035117<\/p>\n<p>&nbsp;<\/p>\n<p>Kabbani M, et al. Human hepatocyte PNPLA3-148M exacerbates rapid non-alcoholic fatty liver disease development in chimeric mice. Cell Rep 2022, 40(11):111321. Doi: 10-1016-j.celrep.2022.111321<\/p>\n<p>&nbsp;<\/p>\n<p>Yu Y, et al. An RNA-based system to study hepatitis B virus replication and evaluate antivirals. Sci Adv 2023, 9(15).eadg6265. doi: 10-1126\/sciadv.adg6265<\/p>\n<p>&nbsp;<\/p>\n<p>Yu Y, et al. Deep mutational scanning of hepatitis B virus reveals a mechanism for <em>cis<\/em>-preferential reverse transcription. Cell 187(11):2735-2745. doi:10-1016\/j.cell.2024.04.008<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<h3>Week 2<\/h3>\n<p><b><span data-contrast=\"auto\">9:00-10:30h &#8211; Theoretical class VII \u2013T-cell priming to mycobacteria I: Dendritic cells and BCG<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr. Antonio Rothfuchs \u2013 Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">&nbsp;<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\"><span class=\"TextRun SCXW105790367 BCX0\" lang=\"EN-US\" xml:lang=\"EN-US\" data-contrast=\"auto\"><span class=\"NormalTextRun SCXW105790367 BCX0\">Day 6 (11\/04\/2024)<\/span><\/span><span class=\"EOP SCXW105790367 BCX0\" data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/span><\/b><\/p>\n<p>11:00-12:00h \u2013 Protocol Discussion V \u2013 CFSE-based migration assay (skin-draining lymph nodes)<span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr. Veronika Krmesk\u00e1, PhD &#8211; Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">&nbsp;<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">13:30-18:00h \u2013 Practical class V \u2013Preparation for practical (CFSE-based migration assay)<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr. Veronika Krmesk\u00e1, PhD &#8211; Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">&nbsp;<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">Day 7 (11\/05\/2024)<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">&nbsp;<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">09:00-10:00h \u2013 Protocol Discussion VI \u2013 Analysis of mouse lymph node by flow cytometry: Dendritic cell subsets and other phagocytes<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr.<\/span> <span data-contrast=\"auto\">Veronika Krmesk\u00e1, PhD &#8211; Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">&nbsp;<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">10:30-18:00h \u2013 Practical class VI \u2013 Analysis of mouse lymph node by flow cytometry <\/span><\/b><span data-contrast=\"auto\">Dr.Veronika Krmesk\u00e1, PhD &#8211; Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">&nbsp;<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">Day 8 (11\/06\/2024)<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">&nbsp;<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">10:00-12:00h \u2013 Practical class VII \u2013 Review of analysis and results and analysis Practical V-VI<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr. Antonio Rothfuchs \u2013 Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr. Veronika Krmesk\u00e1, PhD &#8211; Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">&nbsp;<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">13:00-14:30h &#8211; &#8211; Theoretical class VIII \u2013 Defining the role of migratory Dendritic cells in priming T cells to mycobacteria<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr.Veronika Krmesk\u00e1, PhD &#8211; Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">&nbsp;<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">15:00-16:00h \u2013 Theoretical class XI \u2013 T-cell priming to mycobacteria II: tuberculosis<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr. Antonio Rothfuchs \u2013 Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">&nbsp;<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">16:20-17:30h Practical class VIII \u2013 to be defined<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr. Veronika Krmesk\u00e1, PhD &#8211; Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">&nbsp;<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">Day 9 (11\/07\/2024)<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">&nbsp;<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">09:30-12:00h \u2013 Practical class IX \u2013 Antibody-based assay migration assay<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr. Veronika Krmesk\u00e1, PhD &#8211; Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">&nbsp;<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">13:00-15:00 \u2013 Practical class X\u2013 to be defined<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">&nbsp;<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">Day 10 (11\/08\/2024)<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">&nbsp;<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">10:00-11:30h \u2013 Practical class VII \u2013 Review of analysis and results and analysis Practical IX-X<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr. Antonio Rothfuchs \u2013 Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr. Veronika Krmesk\u00e1, PhD &#8211; Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">&nbsp;<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">13:00-14:00h \u2013 General discussion on results and protocols<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr. Antonio Rothfuchs \u2013 Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr. Veronika Krmesk\u00e1, PhD &#8211; Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">&nbsp;<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">14:00-15:00h &#8211; Theoretical class XI \u2013 \u201cMemorias de um Carioca Su\u00e9co\u201d Research experiences from KI<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Dr. Antonio Rothfuchs \u2013 Karolinska Institutet, Stockholm, Sweden<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">&nbsp;<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">16:30h \u2013 18:00h \u2013 Happy hour<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">&nbsp;<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">Suggested reading for the second week of the course: <\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><b><span data-contrast=\"auto\">&nbsp;<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><a href=\"https:\/\/openarchive.ki.se\/xmlui\/bitstream\/handle\/10616\/48054\/Thesis_Veronika_Krmeska.pdf?sequence=1&amp;isAllowed=y\"><span data-contrast=\"none\">https:\/\/openarchive.ki.se\/xmlui\/bitstream\/handle\/10616\/48054\/Thesis_Veronika_Krmeska.pdf?sequence=1&amp;isAllowed=y<\/span><\/a> <span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">&nbsp;<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><a href=\"https:\/\/openarchive.ki.se\/xmlui\/bitstream\/handle\/10616\/45538\/Thesis_Vishnu_Priya_Bollampalli.pdf?sequence=1&amp;isAllowed=y\"><span data-contrast=\"none\">https:\/\/openarchive.ki.se\/xmlui\/bitstream\/handle\/10616\/45538\/Thesis_Vishnu_Priya_Bollampalli.pdf?sequence=1&amp;isAllowed=y<\/span><\/a> <span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">&nbsp;<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Bollampalli VP, Nyl\u00e9n S, Rothfuchs AG. A CFSE-based Assay to Study the Migration of Murine Skin Dendritic Cells into Draining Lymph Nodes During Infection with <\/span><i><span data-contrast=\"auto\">Mycobacterium bovis<\/span><\/i><span data-contrast=\"auto\"> Bacille Calmette-Gu\u00e9rin. J Vis Exp. 2016 Oct 9;(116):54620. doi: 10.3791\/54620.<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Bollampalli VP, Harumi Yamashiro L, Feng X, Bierschenk D, Gao Y, Blom H, Henriques-Normark B, Nyl\u00e9n S, Rothfuchs AG. BCG Skin Infection Triggers IL-1R-MyD88-Dependent Migration of EpCAMlow CD11bhigh Skin Dendritic cells to Draining Lymph Node During CD4+ T-Cell Priming. PLoS Pathog. 2015 Oct 6;11(10):e1005206. doi: 10.1371\/journal.ppat.1005206.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Aggio JB, Krmesk\u00e1 V, Ferguson BJ, Wowk PF, Rothfuchs AG. Vaccinia Virus Infection Inhibits Skin Dendritic Cell Migration to the Draining Lymph Node. J Immunol. 2021 Feb 15;206(4):776-784. doi: 10.4049\/jimmunol.2000928.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Krmesk\u00e1 V, Aggio JB, Nyl\u00e9n S, Wowk PF, Rothfuchs AG. Cyclooxygenase-Derived Prostaglandin E<\/span><span data-contrast=\"auto\">2<\/span><span data-contrast=\"auto\">\u202fDrives IL-1-Independent <\/span><i><span data-contrast=\"auto\">Mycobacterium bovis<\/span><\/i><span data-contrast=\"auto\"> Bacille Calmette-Gu\u00e9rin-Triggered Skin Dendritic Cell Migration to Draining Lymph Node. J Immunol. 2022 Jun 1;208(11):2549-2557. doi: 10.4049\/jimmunol.2100981.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Krmesk\u00e1 V, Shen L, Nyl\u00e9n S, Wowk PF, Rothfuchs AG. BCG infection dose guides dendritic cell migration and T cell priming in the draining lymph node. Scand J Immunol. 2024 Mar;99(3):e13342. doi: 10.1111\/sji.13342.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Rufino de Sousa N, Sandstr\u00f6m N, Shen L, H\u00e5kansson K, Vezozzo R, Udekwu KI, Croda J, Rothfuchs AG. A fieldable electrostatic air sampler enabling tuberculosis detection in bioaerosols. Tuberculosis (Edinb). 2020 Jan;120:101896. doi: 10.1016\/j.tube.2019.101896.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p><span data-contrast=\"auto\">Rufino de Sousa N, Margerie L, Steponaviciute L, Roux J, Kinahan MW, Olsson D, \u00c1sgeirsson H, Udekwu KI, Rothfuchs AG. Detection of active SARS-CoV-2 in cough aerosols from COVID-19 patients. Infect Dis (Lond). 2024 Jul 8:1-9. doi: 10.1080\/23744235.2024.2374307.<\/span><span data-ccp-props=\"{&quot;201341983&quot;:0,&quot;335551550&quot;:6,&quot;335551620&quot;:6,&quot;335559740&quot;:240}\">&nbsp;<\/span><\/p>\n<p>&nbsp;<\/p>\n<h3>Organizers<\/h3>\n<p>&nbsp;<\/p>\n<p>Juliano Bordignon \u2013 Pryscilla Fanini Wowk \u2013 Ana Luiza Pamplona Mosimann[\/vc_column_text][\/vc_tta_section][\/vc_tta_accordion][\/vc_column][\/vc_row][vc_row][vc_column][vc_empty_space][vc_custom_heading text=&#8221;Confira as Not\u00edcias&#8221; font_container=&#8221;tag:h2|font_size:50|text_align:center|line_height:1&#8243; google_fonts=&#8221;font_family:Lato%3A100%2C100italic%2C300%2C300italic%2Cregular%2Citalic%2C700%2C700italic%2C900%2C900italic|font_style:400%20regular%3A400%3Anormal&#8221;][vc_separator color=&#8221;peacoc&#8221; border_width=&#8221;8&#8243; el_width=&#8221;30&#8243;][\/vc_column][\/vc_row][vc_row][vc_column width=&#8221;1\/4&#8243;][vc_single_image image=&#8221;2468&#8243; 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